lipod293 dna transfection reagent (SignaGen)

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SignaGen lipod293 dna transfection reagent
Lipod293 Dna Transfection Reagent, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipod293+dna+transfection+reagent/lipod293/pm40210047-79-5-12
Average 90 stars, based on 1 article reviews

lipod293 dna transfection reagent - by Bioz Stars, 2026-10

90/100 stars

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Transfection:

Article Title: Cooperative activation of PDK1 and AKT by MAPK4 enhances cancer growth and resistance to therapy.
Article Snippet: .. LipoD293 DNA transfection reagent (SignaGen Laboratories, catalog SL100668) was used for DNA transfection. ..

Article Title: MTA1-DT promotes endometrial cancer growth by modulating G2/M-related gene transcription via PURα.
Article Snippet: .. Plasmid transfection was performed using LipoD293 DNA Transfection Reagent (Cat #: SL100668; SignaGen Laboratories, Rockville, Maryland, USA), while siRNA transfection was conducted using X-tremeGENETM siRNA Transfection Reagent (Cat #: 4476093001, Roche, Basel, Swiss Confederation). .. Total protein was extracted from cells using SDS lysis buffer (Cat #: P0013G; Beyotime Biotechnology,) supplemented with phosphatase and protease inhibitors (Cat #: P1045; Beyotime Biotechnology).

Article Title: MRAP2 inhibits β-arrestin recruitment to the ghrelin receptor by preventing GHSR1a phosphorylation
Article Snippet: .. Cells were transfected with LipoD293 DNA Transfection Reagent from SignaGen laboratory (catalog no.: #SL100668). .. The 3HA-GHSR1a plasmid was obtained from the Missouri S&T cDNA Resource Center.

Article Title: Pkd1 Mutation Has No Apparent Effects on Peroxisome Structure or Lipid Metabolism
Article Snippet: The expression plasmids for enhanced GFP (EGFP)–SLK (pEGFP-C11SKL) and PEX3-GFP (pERB264) were produced by Jay Brenman (Addgene plasmid number 53450; http://n2t.net/addgene:53450; RRID: Addgene_53450) (19) and Michael Lampson (Addgene plasmid number 67764; http://n2t.net/addgene:67764; RRID:Addgene_67764) (20), respectively. .. Cells were seeded on glass coverslips (H-22-1.5-pdl; Neuvitro) or culture dishes with a polymer coverslip bottom (81156; ibidi), and then the cells were transfected with plasmids using a LipoD293 DNA transfection reagent (SL100668; SignaGen Laboratories) for 24–48 hours before the imaging studies. ..

Article Title: A KSHV RNA-binding protein promotes FOS to inhibit nuclease AEN and transactivate RGS2 for AKT phosphorylation.
Article Snippet: .. For dual-luciferase assays, 1 × 105 of HEK293T cells seeded in a 24-well plate were co-transfected with individual Firefly luciferase reporter plasmid together with a Renilla luciferase plasmid, pRL-TS (35, 96), using LipoD293 DNA transfection reagent (SignaGen Laboratories) in triplicates. .. Dual-luciferase reporter assays (Promega) were performed according to the manufacturer’s instructions.

Article Title: Pkd1 Mutation Has No Apparent Effects on Peroxisome Structure or Lipid Metabolism
Article Snippet: The expression plasmids for enhanced GFP (EGFP)–SLK (pEGFP-C1+SKL) and PEX3-GFP (pERB264) were produced by Jay Brenman (Addgene plasmid number 53450; http://n2t.net/addgene:53450 ; RRID:Addgene_53450) ( 19 ) and Michael Lampson (Addgene plasmid number 67764; http://n2t.net/addgene:67764 ; RRID:Addgene_67764) ( 20 ), respectively. .. Live Cell Imaging Cells were seeded on glass coverslips (H-22-1.5-pdl; Neuvitro) or culture dishes with a polymer coverslip bottom (81156; ibidi), and then the cells were transfected with plasmids using a LipoD293 DNA transfection reagent (SL100668; SignaGen Laboratories) for 24–48 hours before the imaging studies. .. Long Chain Fatty Acids Assay by Liquid Chromatography–Mass Spectrometry High-performance liquid chromatography (LC)–grade solvents were purchased from Sigma-Aldrich.

Article Title: Free-fatty acid receptor-1 (FFA1/GPR40) promotes papillary RCC proliferation and tumor growth via Src/PI3K/AKT/NF-κB but suppresses migration by inhibition of EGFR, ERK1/2, STAT3 and EMT.
Article Snippet: .. To assess the role of β-arrestin, cells in 100 mm dishes were transfected with pcDNA3-β-arrestin-(319–418) (kind gift from Dr. Jeffrey Benovic, Thomas Jefferson University) or pcDNA3-β-arrestin-2-YFP (kind gift from Dr. Michel Bouvier, University of Montreal) using 2 μg of the respective plasmid and LipoD293 DNA transfection reagent (Signagen Laboratories, Fredrick, MD), according to the manufacturer’s instructions. .. Total RNA from RCC cells was extracted using Trizol reagent (Invitrogen, Carlsbad, CA) with deoxyribonuclease I (Invitrogen) and first-strand cDNA was synthesized using Omniscript (Qiagen, Germantown, MD) according to the manufacturer’s instructions.

Plasmid Preparation:

Polymer:

Article Title: Pkd1 Mutation Has No Apparent Effects on Peroxisome Structure or Lipid Metabolism
Article Snippet: The expression plasmids for enhanced GFP (EGFP)–SLK (pEGFP-C1+SKL) and PEX3-GFP (pERB264) were produced by Jay Brenman (Addgene plasmid number 53450; http://n2t.net/addgene:53450 ; RRID:Addgene_53450) ( 19 ) and Michael Lampson (Addgene plasmid number 67764; http://n2t.net/addgene:67764 ; RRID:Addgene_67764) ( 20 ), respectively. .. Live Cell Imaging Cells were seeded on glass coverslips (H-22-1.5-pdl; Neuvitro) or culture dishes with a polymer coverslip bottom (81156; ibidi), and then the cells were transfected with plasmids using a LipoD293 DNA transfection reagent (SL100668; SignaGen Laboratories) for 24–48 hours before the imaging studies. .. Long Chain Fatty Acids Assay by Liquid Chromatography–Mass Spectrometry High-performance liquid chromatography (LC)–grade solvents were purchased from Sigma-Aldrich.

Imaging:

Article Title: Pkd1 Mutation Has No Apparent Effects on Peroxisome Structure or Lipid Metabolism
Article Snippet: The expression plasmids for enhanced GFP (EGFP)–SLK (pEGFP-C1+SKL) and PEX3-GFP (pERB264) were produced by Jay Brenman (Addgene plasmid number 53450; http://n2t.net/addgene:53450 ; RRID:Addgene_53450) ( 19 ) and Michael Lampson (Addgene plasmid number 67764; http://n2t.net/addgene:67764 ; RRID:Addgene_67764) ( 20 ), respectively. .. Live Cell Imaging Cells were seeded on glass coverslips (H-22-1.5-pdl; Neuvitro) or culture dishes with a polymer coverslip bottom (81156; ibidi), and then the cells were transfected with plasmids using a LipoD293 DNA transfection reagent (SL100668; SignaGen Laboratories) for 24–48 hours before the imaging studies. .. Long Chain Fatty Acids Assay by Liquid Chromatography–Mass Spectrometry High-performance liquid chromatography (LC)–grade solvents were purchased from Sigma-Aldrich.

Luciferase:

Live Cell Imaging:

Article Title: Pkd1 Mutation Has No Apparent Effects on Peroxisome Structure or Lipid Metabolism
Article Snippet: The expression plasmids for enhanced GFP (EGFP)–SLK (pEGFP-C1+SKL) and PEX3-GFP (pERB264) were produced by Jay Brenman (Addgene plasmid number 53450; http://n2t.net/addgene:53450 ; RRID:Addgene_53450) ( 19 ) and Michael Lampson (Addgene plasmid number 67764; http://n2t.net/addgene:67764 ; RRID:Addgene_67764) ( 20 ), respectively. .. Live Cell Imaging Cells were seeded on glass coverslips (H-22-1.5-pdl; Neuvitro) or culture dishes with a polymer coverslip bottom (81156; ibidi), and then the cells were transfected with plasmids using a LipoD293 DNA transfection reagent (SL100668; SignaGen Laboratories) for 24–48 hours before the imaging studies. .. Long Chain Fatty Acids Assay by Liquid Chromatography–Mass Spectrometry High-performance liquid chromatography (LC)–grade solvents were purchased from Sigma-Aldrich.

other:

Article Title: Pkd1 Mutation Has No Apparent Effects on Peroxisome Structure or Lipid Metabolism
Article Snippet: Prepared samples were applied to 4%–12% gradient Bris-Tris gels (NP0335; Thermo Fisher Scientific) and electrophoresed.

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